MINISTRY OF ENVIRONMENT & FORESTS

NOTIFICATION
New Delhi, the 5th December, 1989
RULES FOR THE MANUFACTURE, USE, IMPORT, EXPORT AND STORAGE OF HAZARDOUS MICRO ORGANISMS GENETICALLY ENGINEERED ORGANISMS OR CELLS
(To be notified under the EP Act, 1986)

G.S.R. 1037(E).-In exercise of the powers conferred by sections 6, 8 and 25 of the Environment (Protection) Act, 1986 (29 of 1986) and with a view to protecting the environment, nature and hea11h, in connection with the application of genetechnology and micro-organisms, the Central Government hereby makes the following rules, namely:-

1. Short title, extent and commencement

(1) These rules may be called the Rules for the Manufacture, Use, Import, Export and Storage of Hazardous micro-organisms Genetically engineered organisms or cells.

(2) These rules shall come into operation on the date to be notified for this purpose in the Official Gazette.

2. Application

(1) These rules are applicable to the manufacture import and storage of micro-organisms and Gene-Technological products.

(2) These shall apply to genetically engineered organisms micro-organisms and cells and correspondingly to any substances and products and food stuffs, etc. of which such cells, organisms or tissues hereof form part.

(3) These rules shall also apply to new genetechnologies apart from those referred to in clauses (ii) and (iv) of rule 3 and these rules shall apply to organisms/micro-organisms and cells generated by the utilisation of such other gene-technologies and to substances and products of which such organisms and cells form part.

(4) These rules shall be applicable in the following specific cases;

    (a) sale, offers for sale, storage for the purpose of sale, offers and any kind of handling over with or without a consideration;

    (b) exportation and importation of genetically engineered cells or organisms;

    (c) production, manufacturing, processing, storage, import, drawing off, packaging and repacking of the Genetically Engineered Products;

    (d) Production, manufacture etc. of drugs and pharmaceuticals and food stuffs distilleries and tanneries, etc. which make use of micro-organisms genetically engineered micro-organisms one way or the other.

(5) These rules shall be applicable to the whole of India.

3. Definition

In these rules unless the context requires,

    (i) "Biotechnology" means the application of scientific and engineering principles to the processing of materials by biological agents to produce goods and services;

    (ii) "Cell hybridisation" means the formation of live cells with new combinations of genetic material through the fusion of two or more cells by means of methods which do not occur naturally;

    (iii) "Gene Technology" means the application of the gene technique called genetic engineering, include self-cloning and deletion as well as cell hybridisation;

    (iv) "Genetic engineering" means the technique by which heritable material, which does not usually occur or will not occur naturally in the organism or cell concerned, generated outside the organism or the cell is inserted into said cell or organism. It shall also mean the formation of new combinations of genetic material by incorporation of a cell into a host cell, where they occur naturally (self cloning) as well as modification of an organism or in a cell by deletion and removal of parts of the heritable material;

    (v) "microorganisms" shall include all the bacteria, viruses, fungi, mycoplasma, cells lines, algae, protodones and nematotes indicated in the schedule and those that have not been presently known to exist in the country or not have been discovered so far.

4. Competant Authorities

(1) Recombinant DNA Advisory Committee (IXDAC)

This committee shall review developments in Biotechnology al national and international levels and shall recommend suitable and-appropriate safety regulations for India in recombinant research, use and applications from time to time. The committee shall function in the Department of Biotechnology.

(2) Review Committee on Genetic Manipulation (RCGM).

This committee shall function in the Department of Biotechnology to monitor the safety related aspect in respect of on-going research projects and activities involving genetically engineered organisms/hazardous microorganisms. The Review Committee on Genetic Manipulation shall include representatives of (a) Department of Biotechnology (b) Indian Council of Medical Research (c) Indian Council of Agricultural Research (d) Council of Scientific and Industrial Research

(e) other experts in their individual capacity. Review Committee on Genetic Manipulation may appoint sub groups.

It shall bring out Manuals of guidelines specifying procedure for regulatory process with respect to activities involving genetically engineered organisms in research use and applications including industry with a view to ensure environmental safety. All ongoing projects involving high risk category and controlled field experiments shall be reviewed to ensure that adequate precautions and containment conditions are followed as per the guidelines.

The Review Committee on Genetic Manipulation shall lay down procedures restricting or prohibiting production sale importation and use of such genetically engineered organisms of cells as are mentioned in the Schedule.

(3) Institutional Biosafety Committee (IBSC).

This committee shall be constituted by an occupier or any person including research institutions handling microorganisms/genetically engineered organisms. The committee shall comprise the Head of the Institution Scientists engaged in DNA work a medical expert and a nominee Of the Department of Bioechology. The occupier or any person including research institutions having microorganisms/genetically engineered organisms shall prepare A he assistance of the Institutional Biosafety Committee (IBSC) an uptodate on-site emergency plan according to the manuals/guidelines of the RCGM and make available copies to the District Level Committee/State Biotechnology Co-ordinating Committee and the Genetic Engineering Approval Committee.

(4) Genetic Engineering Approval Committee (GE.AC)

This committee shall function as a body under the Department of Environment Forests and Wildlife for approval of activities involving large scale use of hazardous microorganisms and recombinants in research and industrial production from the environmental angle. The Committee shall also bc responsible for approval of proposals relating to release of genetically engineered organisms and products into the environment including experiment Field trials.

The composition of the Committee shall be

    (i) Chairman-Additional Secretary Department of Environment Forests and Wild life

    Co-Chairman Representative of Department of Bio-technology

    (ii) Members: Representatives of concerned Agencies and departments namely Ministry of Industrial Development, Department of Biotechnology and the Department of Atomic Energy.

    (iii) Expert members: Director General-Indian Council of Agricultural Research, Director General-Indian Council of Medical Research, Director General-Council of Scientific and Industrial Research, Director General Health Services, Plant Protection Adviser, Directorate of Plant Protection, Quarantine and storage, Chairman, Central Pollution Control Board and three outside experts in individual capacity.

    (iv) Member Secretary: An official of the Department of Environment, Forest and Wildlife.

The Committee may co-opt other members/experts as necessary.

The committee or any person/s authorised by it shall have powers to take punitive actions under the Environment (Protection) Act.

(5) State Biotechnology Co-ordination Committee (SBCC).

There shall be a State Biotechnology Coordination Committee in the States wherever necessary. It shall have powers to inspect, investigate and take punitive action in case of violations of statutory provisions through the Nodal Department and the State Pollution Control Board/Directorate of Health/Medical Services. The Committee shall review periodically the safety and control measures in the various industries/institutions handling genetically engineered Organisms/Hazardous microorganisms. -The compositions of the Coordination Committee shall be:

(i) 	Chief Secretary 					- Chairman
(ii) 	Secretary, Department of Environment			- Member Secretary
(iii) 	Secretary, Department of Health				- Member
(iv) 	Secretary, Department of Agriculture			- Member
(v) 	Secretary, Department of Industries and Commerce	- Member
(vi) 	Secretary, Department of Forests			- Member
(vii) 	Secretary, Department of Public Works/Chief Engineer, 
	Department of Public Health Engineering. 		- Member
(viii) 	State Microbiologists and Pathologists			- Member
(ix) 	Chairman of State Pollution Control Board

The Committee may co-opt other members/experts as necessary.

(6) District Level Committee (DLC)

There shall be a District Level Biotechnology Committee (DLC) in the districts wherever necessary under the District Collectors to monitor the safety regulations in installations engaged in the use of genetically modified organisms/ hazardous microorganisms and its applications in the environment.

The District Level Committee/or any other person/s authorised in this behalf shall visit the installation engaged in activity involving genetically engineered organisms, hazardous microorganisms, formulate information chart, find out hazards and risks associated with each of these installations and coordinate activities with a view to meeting any emergency. They shall also prepare an off-site emergency plan. The District Level Committee shall regularly submit its report to the State Biotechnology Co-ordination Committee/Genetic Engineering Approval Committee.

The District Level Committee shall comprise of:-

(i) District Collector							- Chairman
(ii) Factory Inspector							- Member
(iii) A representative of the Pollution Control Board			- Member
(iv) Chief Medical Officer (District Health Officer) 			- Member (Convenor)
(v) District Agricultural Officer					- Member
(vi) A representative of the Public Health Engineering Department	- Member
(vii) District Microbiologists/Pathologist (technical expert) 		- Member
(viii) Commissioner Municipal Corporation				- Member

The Committee may co-opt other members/experts as necessary.

5. Classification of microorganisms or genetically engineered product

(1) For the purpose of these rules, microorganisms or genetically engineered organisms, products or cells shall be dealt with under two major heads; animal, pathogens and plant pests and these shall be classified in the manner specified in the Schedule.

(2) If any of the microorganisms, genetically engineered organism or cell falls within the limits of more than one risk class as specified in the Schedule, it shall be deemed to belong exclusively to the last in number of such classes.

6. Microorganisms laid down in the Schedule are divided into the following:-

(i) Bacterial Agents;
(ii) Fungal Agents;
(iii) Parasitic Agents;
(iv) Viral, Rickettsial and Chlamydial Agents;
(v) Special Category.

7. Approval and Prohibitions etc.

(1) No person shall import, export, transport, manufacture, process, use or sell any hazardous microorganisms of genetically engineered organisms/substances or cells except with the approval of the Genetic Engineering Approval Committee.

(2) Use of pathogenic microorgnisms or any genetically engineered organisms or cells for the purpose of research shall only be allowed in laboratories or inside laboratory area notified by the Ministry of Environment and Forests for this purpose under the Environment (Protection) Act, 1986.

(3) The Genetic Engineering Approval Committee-shall give directions to the occupier to determine or take measures concerning the discharge of microorganisms/genetically engineered organisms or cells mentioned in the Schedule from the laboratories, hospitals and other areas including prohibition of such discharges and laying down measures to be taken to prevent such discharges.

(4) Any person operating or using genetically engineered organisms/microogranisms mentioned in the schedule for scale up or pilot operations shall have to obtain licence issued by the Genetic Engineering Approval Committee for any such activity. The possessor shall have to apply for licence in prescribed proforma.

(5) Certain experiments for the purpose of education within the field of gene technology or microorganism may be carried out outside the laboratories and laboratory areas mentioned in sub-rule (2) and will be looked after by the Institutional Biosafety Committee.

8. Production

Production in which genetically engineered organisms or cells or micro- organisms are generated or used shall not be commenced except with the consent of Genetic Engineering Approval Committee with respect of discharge of genetically engineered organisms or cells into the environment. This shall also apply to production taking place in connection with development, testing and experiments where such production, etc., is not subject to rule 7.

9. Deliberate or unintentional release

(1) Deliberate or unintentional release of genetically engineered organisms/hazardous microorganisms or cells, including deliberate release for the purpose of experiment shall not be allowed.

Note: Deliberate release shall mean any intentional transfer of genetically engineered organisms/hazardous, microorganisms or cells to the environment or nature, irrespective of the way in which it is done.

(2) The Genetic Engineering Approval Committee may in special cases give approval of deliberate release.

10. Permission and approval for certain substances

Substances and products, which contain genetically engineered organisms or cells or microorganisms shall not be produced, sold, imported or used except with the approval of Genetic Engineering Approval Committee.

11. Permission and approval for food stuffs

Food stuffs, ingredients in food stuffs and additives including processing and containing or consisting of genetically engineered organisms or cells, shall not be produced, sold, imported or used except with thc approval of the Genetic Engineering Approval Committee.

12. Guidelines

(1) Any person who applies for approval under rules 8-11 shall, as determined by the Genetic Engineering Approval Committee submit information and make examinations or cause examinations to be made to eradicate the case, including examinations according to specific directions and at specific laboratories. He shall also make available an on-site emergency plan to GEAC before obtaining the approval. If the authority makes examination itself, it may order the applicant to delay the expenses incurred by it in so doing.

(2) Any person to whom an approval has been granted under rules 8-11 above shall notify the Genetic Engineering Approval Committee of any change in or addition to the information already submitted

13. Grant of approval

(1) In connection with the granting of approval under rules 8 to ll above, terms and conditions shall be stipulated, including terms and conditions as to the control to be exercised by the applicant, supervision, restriction on use, the layout of the enterprise and as to the submission of information to the State Biotechnology Co-ordination Committee or to the District Level Committee.

(2) All approvals of the Genetic Engineering Approval Committee shall be for a specific period not exceeding four year at the first instance renewable for 2 years at a time. The Genetic Engineering Approval Committee shall have powers to revoke such approval in the following situations:-

    (a) If there is any new information as to the harmful effects of the genetically engineered organisms or cells.

    (b) If the genetically engineered organisms or cells cause such damage to the environment, nature or health as could not be envisaged when the approval was given, or

    (c) Non compliance of any condition stipulated by Genetic Engineering Approval Committee.

14. Supervision

(1) The Genetic Engineering Approval Committee may supervise the implementation of the terms and conditions laid down in connection with the approvals accorded by it.

(2) The Genetic Engineering Approval Committee may carry out this supervision through the State Biotechnology Coordination Committee or the State Pollution Control Boards/District Level Committee or through any person authorised in this behalf.

15. Penalties

(1) If an order is not complied with, the District Level Committee or State Biotechnology Co-ordination Committee may take measures at the expense of the person who is responsible.

(2) In case where immediate intervention is required in order to prevent any damage to the environment, nature or health, the District level Committee or State Biotechnology Coordination Committee may take the necessary steps without issuing any order or notice. The expenses incurred for this purpose will be repayable by the person responsible for such damage.

(3) The State Biotechnology Co-ordination Committee/District Level Committee may take samples for a more detailed examination of organisms and cells.

(4) The State Biotechnology Co-ordination Committee/District Level Committee shall be competent to ask for assistance from any other government authority to carry out its instructions.

16. Responsibility to notify interruptions or accidents

(1) Any person who under rule 7-11 is responsible for conditions or arrangements shall immediately notify the District Level Committee/State Biotechnology Co-ordination Committee and the state medical officer of any interruption of operations or accidents shat may lead to discharges of genetically engineered organisms or cells which may be harmful to the environment, nature or health or involve any danger thereto.

(2) Any notice given under sub-rule (I) above shall not lessen the duty of the person who is responsible to try effectively to minimise or prevent the effects of interruptions of operations or accidents.

17. Preperation Off-site emergency Plan by the DLC

(1) It shall be the duty of the DLC to prepare an off-site emergency plan detailing how emergencies relating to a possible major accident at a site will be dealt with and in preparing the plan, the DLC shall consult the occupier and such other person as it may deem necessary.

(2) For the purpose of enabling the DLC- to prepare. the emergency plan required under sub-rule (1), the occupier shall provide the DLC with such information relating to the handling of hazardous microorganisms/ genetically engineered organisms under his control as the DLC may required including the nature, extent and likely off-site affects of a possible major accident and the DLC shall provide the occupier with any information from the off-site emergency plan which relates to his duties under rule 16.

18. Inspections and informations regarding finance

(1) The State Biotechnology Co-ordination Committee or the Genetic Engineering Approval Committee/the DLC or any person with special knowledge duly authorised by the State Biotechnology Co-ordination Committee or the Genetic Engineering Approval Committee or the DLC where it is deemed necessary, at any time on due production of identity be admitted to public as well as to private promises and localities for the purpose of carrying out supervision.

(2) Any person who is responsible for activities subject to rules 7-11 above shall at the request of District level Committee or State Biotechnology Coordination Committee or the GEAC submit all such information including information relating to financial conditions and accounts, as is essential to the authority's administration under these rules He shall also allow supervision or inspection by the authorities or persons indicated in sub-rule (1).

(3) The Genetic Engineering Approval Committee may fix fees to cover, in whole or in part, the expenses incurred by the authorities in connection with approvals, examinations, supervisions and control.

19. Appeal

(l) Any person aggrieved by a decision made by Genetic Engineering Approval Committee/State Biotechnology Co-ordination Committee in pursuance of these rules may within thirty days from the date on which the decision is communicated to him, prefer an appeal to such authority as may be appointed by Ministry of Environment and Forests provided that the appellate authority may entertain the appeal after the expiry of the said period of thirty days if such authority is satisfied that the appellant was prevented by sufficient cause from filing the appeal in time.

20. Exemption

The Ministry of Environment and Forests shall, wherever necessary, exempt an occupier handling a particular microorganism/genetically engineered organism from rule 7-11.

ANIMAL AND HUMAN PATHOGENS
SCHEDULE
BACTERIAL
Risk Group II
    Acinetobacter calcoacetieus
    Actinobacillus all species except A mallei, which in Risk Group III
    Acromonoas hydrophila
    Arzona hinshawii-all serotypes
    Baciluss anthracis
    Bordetella-all species
    Borrelia recurrentis B. vincenti
    Campylobacter fetus
    Camphylobacter jejuni
    Chalarnydia psittaci
    Cheamydia trachomatis
    Clostridium hauvoci, Cl, Difficle Cl. fallax, Clhaemolyticum Cl histolyticum, Cl, novyi, (Cl. perfringes), Cl. speticum, Cl. sordeili
    Cornylebacterium diptheriae, C. equi, C. haemolyticum, C. pseudo tuberculosis, C. pyogenes, C. renale
    Diplococcus (Streptococcus) pneumoniae
    Edwardsiila tarda
    Erysipelothix insidiosa
    Escherichia Coli-all enteropathogenic serotypes enterotaxigenic
    Haemophilus ducrevi, H. influenzae, H. pneumoniae
    Herellea vaginicola
    Klebsiella- all species and all serotypes
    Legionlla pneumophila
    Letionella
    Leptospira intenogans all serotypes reported in India
    Listeria, all species
    Mima polymorpha
    Moraxella-All species
    Mycobacteria-all species including Mycobacterium avium
    M. bovis M. tuberculosis, M. lepreae
    Mycoplasma-all species except M. mycoides and M. angalactiae
    Neisseric gonorrhoea, N. meningitis
    Pasteurella all species except those listed in Risk Group III
    Salmonella-all species and all serotypes
    Shigella all species and all serotypes
    Shpacrophorus necrophorus
    Staphylococcus aureus
    Streptobafcillus moniformis
    Strptococcus pneumoniae
    Streptococcus pyogenes, S. equi
    Streptomyces madurae, s. pelleteri, s. somaliensis
    Treponema carateum, T. palidum and T. pertenue
    Vibrio foctus, V. comma including biotype EI Tor and
    V. parahemolyticus.
    Vibrio cholerae

Risk Group III:

    Actinobacillus mallei
    Baltenella-all species
    Brucella all species
    Clostridium botulium, Cl. tetani
    Francisella tularensis
    Mycobacterium avium, M. bovis, M. tuberculosis, m. leprae
    Paseturella multocida type B ("buffalo" and other foreign virulent strains)
    Pseudomonas pseudomallai
    Yersinia pestis

FUNGAL
Risk Group II

    Actinomycetes (including Nocardia Sp, Actinomyces species and Arachina prpinica)
    Aspergilus fumigatus
    Blastomyces dermatitis
    Cryptococcus neoformans C. fersiminosos
    Epidermophyton madurella, microsporon
    Paracoccidiodes brasiliensis
    Sporothrix
    Trichodenna
    Trichophylon

Risk Group III

    Coccidioaes immitis Histoplasma capulatum
    Histoplasma capsulalum var duboissl

PARASITIC
Risk Group II

    Entahoe'oa histolytica
    Leishmania species
    Naegeleria gruberia
    Plasmodium theilera, P. babesia P. falcoparum
    Plasmodium babesia
    Schistosoma
    Toxoplasma gondii
    Toxocara canis
    Trichinella spiralis
    Trichomanas
    Trypanosoma cruzi

Risk Group III

    Schisistosoma mansoni

VIRAL RICKEITSIAL AND CHAIMYDIAL
Risk Group II

    Adenoviruses-Human, all types
    Avian loukosis
    Cache Valley virus
    CELO (avain adenovirus)
    Cosackie A and B viruses
    Corona viruses
    Cytomegalo viruses
    Dengue virus, when used for transmission experiments
    Echo viruses-all types
    Encephalomyocarditis virus (EMC)
    Flanders virus
    Hart Past virus
    Hepatitis-associated antigen material-hepatitis A and B viruses, non A and non B, HDV
    Herpes viruses-except herpes virus simiae (monkey B virus) which is in Risk Group IV.
    Infections Bovine Rhinotraechitis virus (IBR).
    Infections Bursal diesaes of poultry and Infectious Bronchitus
    Infections Laryngotraechitis (ILT)
    Influex za virus-all types, except A/PR8/34 which in Risk Group I
    Langat virus Leucosis Complex
    Lymphogranuloma venereum agent
    Mark's Disease virus
    Measles virus
    Mumps virus
    Newcastle disease virus (other than licenced strain for vaccine use)
    Parainfluenza viruses-all types except parainfluenza virus 3, SF4 strain, which is in Risk Group I
    Polio viruses-all types wild and attenuated
    Poxvirus-all typcs except Mastrim, monkey pox, sheep pox and white pox, which depending on experiments are in Risk Group III or IV.
    Rabies virus-all strains except rabies stret virus, which should be classified in Risk Group III when inoculated into comivores
    Reoviruses-all types
    Respiratory syncital virus
    Rhinoviruses-all types
    Rinderpest (other than vaccine strain in use)
    Rubella virus
    Stimian virues-all types except herpeavirus simae (Monkey Virus) which is in Risk Group IV.
    Simian virus 4()
    Ad 7 SV 40 (defective)
    Sindbis virus
    Tensaw virus
    Turlock virus
    Vaccinia virus
    Varicella virus
    Vole rickettsia
    Yellow fever virus, 17 D vaccine strain

Risk Group III

    African House Sickness (attenuated strain except animal passage)
    Alastrim, monkey pox and whitepox, when used into vitro
    Arboviruses-All strains except those in Risk Group II and IV
    Blue tongue virus (only serotypes reported in India)
    Ebola fever Virus
    Epstiein-Barr virus
    Feline Leukemia
    Feline sarcoma
    Foot and Mouth Disease virus (all seritypes and subtypes)
    Gibbon Apc Lymphosarcoma
    herpesvirus atcles
    herpesirus saimiri
    herpes Simplex 2
    HIV-1 & HIV-2 and strains of SIV
    Infcctious Equine Anaemia
    Lymphocylic choriomeningitis virus (LCM)
    Monkey pox, when used in vitro
    Non-defective Adeno-2 SV-40 hybrids
    Psettacosis-ornithosis-trachoma group of agents
    Pscudorabies virus
    Rabies street virus, when used inoculations of carnivores
    Rickltsia-all species except Vole rickeitsia on Coxiell burnetti when used for vector transmission or animal inoculatin experiments
    Sheep pox (field strain)
    Swine Fever virus
    Vesiculat stomatitis virus
    Woolly monkey Fibrosarcoamm
    Yaba pox virus

Risk Group IV

    Alastrim, monkeypox, whitepox, when used for transmission or animal inoculation experiments

    Hemorrhagic fever agents, including Crimean hemorrhagic fever (congo)

    Korean hemorrhagic fever and others as yet undefiened

    Herpesvirus simlae (monkey B virus)

    Tick-borne encephalitis virus complex, including-Russian Spring Summer Encephalif s, Kyasanur Forest Diseast, omsk hemorrhagic fever and Central European encephalitis viruses

SPECIAL CATEGORY

BACTERIAL

    Contagious Equine Metritis (H. equinilais) Pestis-petit de ruminantium

VIRAL RICKE1TSIAL AND CHLAMYDIAL:

    African Horse Sickness virus (serotypes not reported in Indian and challenge strains)
    African Swine Fever
    Bat ratiesvirus
    Blue tongue virus (serotypes not reported in India)
    Exoitic FMD virus types and sub-types
    Junin and Machupo viruses
    Lassa virus
    Marburg virus
    Murrey valley encephalitis virus
    Rift Valley Fever virus
    Smallpox virus-Archieval storage and propagation Swine Vesicular Disease
    Veneseulan equine encephalitis virus epidemic strains
    Western Equine encephalitis virus
    Yellow fever virus-Wild strain
    Other Arboviruses causing eizootics and so far not recorded in India.

B: PLANT PESTS

Any living stage (including active and dormant forms) of insects, mites, nematodes, slugs, snails, bacteria, fungi, protozoa, other parasitic plants or reproductive parts thereof: viruses or any organisms similar to or allied with any of the foregoing; or any infectious agents or substances, which can directly or indirectly injure or cause disease or damage in or to any plants or parts thereof, or any processed, manufactured, or other products of plants are considered plant pests.

Organisms belonging to all lower Taxa contained within the group listed are also included. 1. Viruses
All viruses

Alt bacteria, fungal, algal, plant, insect and neumatode viruses; special care should be taken for-

    (i) Geminiviruses,
    (ii) Calulimoviruscs,
    (iii) Nuclear Polyhedrosis viruses,
    (iv) Grandulosis viruses, and
    (v) Cyloplasmic polyhedrosis viruses

2. Bacteria

    Family Pseudomonadaceae
    Genus Pscudomonas
    Genus Xanthomonas
    Genus Azolobacter

Family Rhizobiaceae

    Genus Rhizobium/Azorhizobium
    Genus Bradyrhizobium
    Genus Agrobacterium
    Genus Phyllobacterium
    Genus Erwinia
    Genus Enterobacter
    Genus Klebzieller

Family Spirollaceae

    Genus Azospirillum
    Genus Acqaspirillum
    Genus Occeonospirillum
    Family Slreplomycetaceae
    Genus Streplomyces
    Genus Nocardia

Family Actionmycetaceas
Genus Aclinomyces

Coryneform Group

    Genus Clavibacter
    Genus Arthrobacter
    Genus Curlobacterium
    Gcnus Bdellovibro
    Family Rickettsiaceae

Rickeltsial like organisms associated with insect diseases

Gram-negative phloem-limited bacteria associated with plant diseases

Gram-negative xylem-limited bacteria associated with plant diseases

Cynobacteria-all members of blue-green algae

Mollicutes

Family Spiroplasmataceae

Mycoplasma-like organisms associated with plant diseases

Mycoplasma-like organisms associated with insect diseases

Algae

    Family Chlomphyceae
    Family Euglenophyceae
    Family Pyrophyceae
    Family Chrysophaceae
    Family Phacphyceae
    Family Rhodophyceae

Fungi
Family Plasmodiophoraceae
Family Chytridiaceae
Family Oldipiopsidaceae
Family Synchytriaceae
Family Catenariaceae
Family Coelomomycetaceae
Family Saprologniaceae
Family Zoopagaceae
Family Albuginaceae
Family Peronosporaceae
Family Pythiaceae
Family Mucoraceae
Family Choanephoraceae
Family Mortierellaceae
Family Endogonaceae
Family Syneephalastraceae
Family Dimargaritaceae
Family Kickxellaceae
Family Saksenaeaceae
Family Entomophthoraceae
Family Eecrinaceae
Family Taphrinaceae
Family Endomycotaceae
Family Saceharamyeetacea
Family Eutoliaceae
Family Gymnoaseaceae
Family Aseophaeriaecae
Family Onygenaceae
Family Microascaceae
Family Protomycetaceae
Family Elsinoeaceae
Family Mynaginaceae
Family Dothidiaceae
Family Chaetothyriaceae
Family Pharmularieaceae
Family Phillipsicllaceae
Family Gysteriaceae
Family Pleosporaceae
Family Melamomataceae
Family Ophiostomataceae
Family Aseosphaeriaceae
Family Erysiphaceae
Family Meliolaceae
Family Xylariaceae
Family Diaporthaceae
Family Hypoercaceae
Family Clavicipataceae
Family Phacidiaceae
Family Ascocorticiaceae
Family Hemiphacidiaceae
Family Dennataceae
Family Selerotimiaceae
Family Cyttariaceae
Family Helosiaceae
Family Sarocostomataceae
Family Sarcoscyphaceae
Family Auricolariaceae
Family Ceratobasidiaceae
Family Corticiaceae
Family Hymenochaetaceae
Family Echiondintiaceae
Family Eistuliniaceae
Family Clavariaceae
Family Polyporaceae
Family Tncholomataceae
Family Ustilaginaceae
Family Sporobolomycetaceae
Family Uredinaceae
Family Agaricaceae
Family Graphiolaceae
Family Pucciniaceae
Family Mclampsoraceae
Family Gandodermataceae
Family Labonlbeniaceae
Family Sphaeropsidaceae
Family Mclabconiaceae
Family Tuberculariaceae
Family Dematiaceae
Family Moniliaceae
Family Aganomucotaceae

Parasitic Weeds

    Family Balanophoraceae-parasitic species
    Family Cuscutaceae-parasitic species
    Family Thydonoraceae-parasilic species
    Family Lauraceae-parasitic species Genus Cassytha
    Family Lennoaceae-parasilic species
    Family Loranlhaceae-parasitic species
    Family Myzodendraceae-parasitic species
    Family Olacaceae-parasitic species
    Family Orobanchaceae-parasitic species
    Family Rafflesiaceae-parasitic species
    Family Santalaceae-parasitic species
    Family Scrophulariaceae-parasitic species

Protozoa

    Genus Phytomonas
    And all Protozoa associated with insect dest

Nematodes

    Family Anguinidae
    Family Belonolaimidae
    Family caloosiidae
    Family Cariconematidae
    Family dolichodoridae
    Family Fergusbbiidae
    Family hemicycliophoridae
    Family Heteroderidae
    Family Hoplolaimidae
    Family Mcloidogynidae
    Family Neotylenchidae
    Family Nolhotylenchaidae
    Family Paralylenchidae
    Family Pralyjenchidae
    Family Tylenchidae
    Family Tylenchulidae
    Family Aphelenchoidae
    Family Longdoridae
    Family Tlichodoridae

Mollusca

    Super family Planorbacca
    Super family Achatinacca
    Super family Arionacca
    Super family Limacacca
    Super family Helicacea
    Super family Vcronicellacea

Arthropoda

    Super family Ascoidea
    Super family Dermanyssoidea
    Super family Erjophyoidea
    Super family Tetranychoidea
    Super family Tetranychoidea
    Super family Eupododca
    Super family Tydcoidea
    Super family Erythraenoidca
    Super family Trombidioidea
    Super family Hydryphantoidea
    Super family Tarasonemoidea
    Super family Pyomotoiodea
    Super family Hcmisaracoptoidea
    Super family Acaroidea
    Order Polydesmida
    Family Sminlhoridao
    Family Forfieulidzo
    Order Isptera
    Order Thysanoptera
    Family Acredidea
    Family Gryllidae
    Family Gryllacridiedae
    Family Gryllotalpidae
    Family Phasmatidao
    Family Ronalcidao
    Family Tettigoniidao
    Family Tatragidao
    Family Thaumastocoridae
    Super family Picsmaloidca
    Super family Lygacoidea
    Super family Idiosloloidea
    Super family Careoidea
    Super family Penalomoidea
    Super family Pyrrhoeomidea
    Super family Tingoidea
    Super family Miroidea
    Order Homoplara
    Family Anobiidae
    Family Apionidae
    Family Anthrididae
    Family Bosirichidae
    Family Brentidae
    Family Bruchidae
    Family Bupresiodae
    Family Byturidae
    Family Caniharidae
    Family Carabidae
    Family Ceambienidae
    Family Chrysomelidae
    Family Coecinellidae
    Family Curculionidae
    Family Dermestidae
    Family Elateridae
    Family Hydrophilidae
    Family Lyctidae
    Family Meloidae
    Family Moredellidae
    Family Platypodiadae
    Family Scarabaeldae
    Family Scolylidae
    Family Selbytidae
    Order Lepidoptera
    Family Agromyidae
    Family Anthomiidae
    Family Cecidomiidae
    Family Chioropidae
    Family Ephydridae
    Family Lonchacidae
    Family Musicdae
    Family Otitidac
    Family Syrphidae
    Family Tephrilidae
    Family Tipulidae
    Family Apidae
    Family Caphidae
    Family Chalcidae
    Family Cynipidae
    Family Eurytomidae
    Family formisidae
    Family Psilidae
    Family Sircidae
    Family Tenthredinidae
    Family Torymidae
    Family Xyloiopidae

and

also unclassified organism and/or organisms whose classification is unknown, and all other organisms associated with plant and insect disease.


Published in the Gazette No. 621 dt. 5-12-89

Corrections made in terms of corrigendum No. G.S.R. 137(E) dt. 21-2-90 published in the Gazette.